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Vector Laboratories vicia villosa agglutinin vva lectinfitc
Vicia Villosa Agglutinin Vva Lectinfitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/L-1230-5/Unconjugated+Vicia+Villosa+Lectin+(VVL%2C+VVA)/pm39844613-63-28-33
Average 93 stars, based on 144 article reviews

vicia villosa agglutinin vva lectinfitc - by Bioz Stars, 2026-11

93/100 stars

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Flow Cytometry:

Article Title: C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter-mediated doxorubicin resistance.
Article Snippet: .. Flow cytometry analysis of lectin binding and cellular apoptosis Cells (1 105) were suspended in 100 μl PBS with 0.5% BSA, treated with neuraminidase (Sigma-Aldrich), and incubated with Vicia villosa agglutinin (VVA) lectinFITC (Vector Laboratories, Newark, CA, USA) to detect O-glycan changes and Annexin V-FITC (Bio Pioneer Tech Co., New Taipei City, Taiwan) to assess apoptosis. .. After washing, the fluorescence intensity of 10,000 cells was analyzed using flow cytometry (BD Pharmingen, Franklin Lakes, NJ, USA).

Binding Assay:

Incubation:

Article Title: Targeted CRISPR Screens Reveal Genes Essential for Cryptosporidium Survival in the Host Intestine
Article Snippet: .. Secondary antibodies were incubated for 1 hour in 1% BSA/PBS along with the fluorescein labelled 1:4000 Vicia villosa lectin (VVL) (Vector Lab) or 1:1000 Helix pomatia agglutinin (HPA) (Invitrogen) that stains parasites. .. Nuclei were stained by incubating with 1:10,000 Hoechst 33342 (Invitrogen) in 1X PBS for 5 minutes.

Article Title: C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter-mediated doxorubicin resistance.
Article Snippet: Detection used HRP-conjugated secondary antibodies and ECL reagents (GE Healthcare, Chicago, IL, USA). .. For lectin pull-down assays, 500 μg of lysate proteins were incubated with VVA lectin-conjugated beads (Vector Laboratories) and analyzed by SDS-PAGE for western blotting. .. Immunofluorescence staining Cells were cultured on chamber slides (SPL Life Sciences, Pocheon-si, South Korea), fixed with 4% paraformaldehyde, permeabilized with 0.25%Triton X100, and blocked with 2% BSA (Bio-Rad).

SDS Page:

Western Blot:

Whole Genome Amplification:

Article Title: Fingerprint Profiling of Glycans on Extracellular Vesicles via Lectin-Induced Aggregation Strategy for Precise Cancer Diagnostics.
Article Snippet: Ovalbumin derived from egg white (Cat#A605084) was purchased from Sangon Biological Engineering Technology & Co. Ltd. PNGase F (Cat#P0704S) was purchased from New England Biolabs (NEB). .. The lectins, including Concanavalin A (ConA, Cat#L- 1000-500), Sambucus Nigra Agglutinin (SNA, Cat#L-1300-5), Lotus Tetragonolobus Lectin (LTL, Cat#L-1320-5), Griffonia Simplicifolia Lectin I (GSL-I, Cat#L-1104-1), Wheat Germ Agglutinin (WGA, Ca #L-1020), Vicia Villosa Agglutinin (VVA, CatL-1230-5) and Ulex Europaeus Agglutinin I (UEA-I, Cat#L-1060), were purchased from Vector Laboratories. ..

Image Search Results

a-b. Relative luminescence units (RLU) from a HCT8 cell monolayer infected for 24 hours with C. parvum parasites that were transiently transfected with a nanoluciferase-mCherry expression vector, trialling multiple electroporation programs ( a ) and excystation reagents used prior to transfection ( b ). TDC (taurodeoxycholate), NaCIO (sodium hypochlorite), TC (taurocholate), RMPI (media), DC (deoxycholate) PBS (phosphate-buffered saline). c . Quantification of the improved transfection efficiency measured by RLU. In black, the standard method, in yellow, the revised method. d. Visualisation of the increased transfection efficiency of the revised method (right panel) compared to the standard method (left panel) using the mCherry-expression vector. Blue (Hoechst), nuclei; green (Vicia villosa lectin (VVL)), parasites; red (mCherry Ab), mCherry/transfected parasites. All data shows the mean ± standard deviation from 2 biological replicates. e. RLU from a HCT8 cell monolayer infected for 24 hours with C. parvum parasites transfected with a repair cassette designed to replace the thymidine kinase gene, using different lengths of homology repair. Data shows the mean ± sd from three technical replicates.

Journal: bioRxiv

Article Title: Targeted CRISPR Screens Reveal Genes Essential for Cryptosporidium Survival in the Host Intestine

doi: 10.1101/2024.11.22.624643

Figure Lengend Snippet: a-b. Relative luminescence units (RLU) from a HCT8 cell monolayer infected for 24 hours with C. parvum parasites that were transiently transfected with a nanoluciferase-mCherry expression vector, trialling multiple electroporation programs ( a ) and excystation reagents used prior to transfection ( b ). TDC (taurodeoxycholate), NaCIO (sodium hypochlorite), TC (taurocholate), RMPI (media), DC (deoxycholate) PBS (phosphate-buffered saline). c . Quantification of the improved transfection efficiency measured by RLU. In black, the standard method, in yellow, the revised method. d. Visualisation of the increased transfection efficiency of the revised method (right panel) compared to the standard method (left panel) using the mCherry-expression vector. Blue (Hoechst), nuclei; green (Vicia villosa lectin (VVL)), parasites; red (mCherry Ab), mCherry/transfected parasites. All data shows the mean ± standard deviation from 2 biological replicates. e. RLU from a HCT8 cell monolayer infected for 24 hours with C. parvum parasites transfected with a repair cassette designed to replace the thymidine kinase gene, using different lengths of homology repair. Data shows the mean ± sd from three technical replicates.

Article Snippet: Secondary antibodies were incubated for 1 hour in 1% BSA/PBS along with the fluorescein labelled 1:4000 Vicia villosa lectin (VVL) (Vector Lab) or 1:1000 Helix pomatia agglutinin (HPA) (Invitrogen) that stains parasites.

Techniques: Infection, Transfection, Expressing, Plasmid Preparation, Electroporation, Saline, Standard Deviation

a. Schematic and genotyping PCR of TK-diCRE parasites. b. TK-diCRE parasites were allowed to infect a HCT8 monolayer and genomic DNA was extracted at 2, 4, 8, 24, 32 and 48 hours post infection in the presence and absence of rapamycin. Diagnostic PCRs confirmed the level of excision that had occurred at the respective timepoint. c-d. TK-diCRE parasites were allowed to infect a HCT8 monolayer in the presence and absence of rapamycin, and the monolayer was fixed and stained at 4, 8, 12, 16, 24, 32 and 48 hours post infection. The HA tag per parasite was quantified by automated imaging, representation images are shown in d ; blue (Hoechst); green (Vicia villosa lectin (VVL)); red (αHA). Data shown is representative of 2 biological replicates, each with 2 technical replicates ± sd. e. Schematic and genotyping PCR of RNR-diCRE parasites. f. EdU assay with quantifications showing newly synthesised DNA between 28 and 32 hours in wildtype, TK- and RNR-diCRE parasites in the presence and absence of rapamycin. Blue (Hoechst); green, (Vicia villosa lectin (VVL)); magenta, (EdU). Data shown is two biological replicates with the number of parasites quantified indicated. Scale bar = 10μm.

Journal: bioRxiv

Article Title: Targeted CRISPR Screens Reveal Genes Essential for Cryptosporidium Survival in the Host Intestine

doi: 10.1101/2024.11.22.624643

Figure Lengend Snippet: a. Schematic and genotyping PCR of TK-diCRE parasites. b. TK-diCRE parasites were allowed to infect a HCT8 monolayer and genomic DNA was extracted at 2, 4, 8, 24, 32 and 48 hours post infection in the presence and absence of rapamycin. Diagnostic PCRs confirmed the level of excision that had occurred at the respective timepoint. c-d. TK-diCRE parasites were allowed to infect a HCT8 monolayer in the presence and absence of rapamycin, and the monolayer was fixed and stained at 4, 8, 12, 16, 24, 32 and 48 hours post infection. The HA tag per parasite was quantified by automated imaging, representation images are shown in d ; blue (Hoechst); green (Vicia villosa lectin (VVL)); red (αHA). Data shown is representative of 2 biological replicates, each with 2 technical replicates ± sd. e. Schematic and genotyping PCR of RNR-diCRE parasites. f. EdU assay with quantifications showing newly synthesised DNA between 28 and 32 hours in wildtype, TK- and RNR-diCRE parasites in the presence and absence of rapamycin. Blue (Hoechst); green, (Vicia villosa lectin (VVL)); magenta, (EdU). Data shown is two biological replicates with the number of parasites quantified indicated. Scale bar = 10μm.

Techniques: Infection, Diagnostic Assay, Staining, Imaging, EdU Assay

a-b. TK-diCRE (shown in green) and ribonucleotide reductase (RNR) -diCRE (shown in purple) parasites were generated and used to infect an HCT8 monolayer in the presence or absence of rapamycin. Genomic DNA was extracted at 6, 24 and 48 hours and diagnostic PCRs confirmed the level of excision at the given time points. c. HCT8 monolayers infected with TK-diCRE and RNR-diCRE parasites in the presence or absence of rapamycin. At 6, 24 and 48 hours the monolayers were fixed, stained and the number of parasites per host nuclei was quantified. Representative images are shown, with nuclei in blue (Hoechst), and parasites in green (Vicia villosa lectin). Data shown is representative of 2 biological replicates ± sd of 4 technical replicates. Significance is determined using a two-tailed unpaired t-test, ns = not significant, ** = p ≤ 0.01. d. TK-diCRE or RNR-diCRE parasites were used to infect Ifnγ -/- mice, which were either treated with rapamycin or vehicle (DMSO) in their drinking water starting at day 2 post infection. Data shown is a biological replicate ± SEM of the 2 technical replicates, n = 2 mice per condition.

Journal: bioRxiv

Article Title: Targeted CRISPR Screens Reveal Genes Essential for Cryptosporidium Survival in the Host Intestine

doi: 10.1101/2024.11.22.624643

Figure Lengend Snippet: a-b. TK-diCRE (shown in green) and ribonucleotide reductase (RNR) -diCRE (shown in purple) parasites were generated and used to infect an HCT8 monolayer in the presence or absence of rapamycin. Genomic DNA was extracted at 6, 24 and 48 hours and diagnostic PCRs confirmed the level of excision at the given time points. c. HCT8 monolayers infected with TK-diCRE and RNR-diCRE parasites in the presence or absence of rapamycin. At 6, 24 and 48 hours the monolayers were fixed, stained and the number of parasites per host nuclei was quantified. Representative images are shown, with nuclei in blue (Hoechst), and parasites in green (Vicia villosa lectin). Data shown is representative of 2 biological replicates ± sd of 4 technical replicates. Significance is determined using a two-tailed unpaired t-test, ns = not significant, ** = p ≤ 0.01. d. TK-diCRE or RNR-diCRE parasites were used to infect Ifnγ -/- mice, which were either treated with rapamycin or vehicle (DMSO) in their drinking water starting at day 2 post infection. Data shown is a biological replicate ± SEM of the 2 technical replicates, n = 2 mice per condition.

Techniques: Generated, Diagnostic Assay, Infection, Staining, Two Tailed Test

a. Schematic and genotyping PCR of Cp23-diCRE parasites. b. Visualising gliding C. parvum sporozoites using super-resolution microscopy. Green (Vicia villosa lectin (VVL) which marks both parasite and trail); red (αCp23); blue (Hoechst). Scale bar = 5 μm. c-e. Cp23-diCRE and wildtype parasites were allowed to infect an HCT8 monolayer in the presence or absence of rapamycin. At 22 hours post infection the life cycle stage was quantified. Data shows 2 biological replicates. Significance is determined using a two-tailed unpaired t-test, ns = not significant, **** = p ≤ 0.0001. Representative images are shown in e . Blue (Hoechst); green (Vicia villosa lectin (VVL)); magenta (phallodin - actin); red (αCp23). Scale bar = 10μm.

Journal: bioRxiv

Article Title: Targeted CRISPR Screens Reveal Genes Essential for Cryptosporidium Survival in the Host Intestine

doi: 10.1101/2024.11.22.624643

Figure Lengend Snippet: a. Schematic and genotyping PCR of Cp23-diCRE parasites. b. Visualising gliding C. parvum sporozoites using super-resolution microscopy. Green (Vicia villosa lectin (VVL) which marks both parasite and trail); red (αCp23); blue (Hoechst). Scale bar = 5 μm. c-e. Cp23-diCRE and wildtype parasites were allowed to infect an HCT8 monolayer in the presence or absence of rapamycin. At 22 hours post infection the life cycle stage was quantified. Data shows 2 biological replicates. Significance is determined using a two-tailed unpaired t-test, ns = not significant, **** = p ≤ 0.0001. Representative images are shown in e . Blue (Hoechst); green (Vicia villosa lectin (VVL)); magenta (phallodin - actin); red (αCp23). Scale bar = 10μm.

Techniques: Super-Resolution Microscopy, Infection, Two Tailed Test

a-b. HCT8 cell monolayers infected with Cp23-diCRE in the presence or absence of rapamycin. At 6, 24 and 48 hours, gDNA was extracted and diagnostic PCRs confirmed the level of excision at the given timepoint. c. HCT8 cell monolayers infected with Cp23-diCRE in the presence or absence of rapamycin. At 6, 24 and 48 hours, the monolayer was fixed and stained and the parasite per host nuclei was quantified. Representative images are shown, with nuclei in blue (Hoechst), and parasites in green (Vicia villosa lectin). Data shown is representative of two biological replicates ± sd of the 4 technical replicates. Significance is determined using a two-tailed unpaired t-test, ns = not significant, * = p ≤ 0.05, ** = p ≤ 0.01 . d. Ifnγ -/- mice were infected with 50,000 Cp23-diCRE parasites and treated with rapamycin or DMSO control in their drinking water at 2 days post infection. Data shown is the mean of a biological replicate ± SEM of the 2 technical replicates, n = 2 mice per condition. e. Super resolution microscopy at 24 hours post infection in vitro with Cp23-diCRE parasites in the presence or absence of rapamycin; green (helix pomatia agglutinin (HPA)), parasite; magenta, (sytox), nuclei; red (Cp23 Ab), Cp23. Scale = 2μm. f. Expansion microscopy of the C. parvum asexual stages: sporozoite, merozoite, trophozoite and meront, and the sexual stages: macrogamont (female) and microgamete (male); green (helix pomatia agglutinin (HPA)); blue (N-hydroxysuccinimide (NHS ester); magenta (sytox); red (αCp23). Scale bar = 5μm. Median expansion factor of 4.5. g. Transmission electron microscopy of an excysted and unexcysted C. parvum sporozoite coupled with immunogold labelling with Cp23 antibody. Scale = 300nm . h. Permeabilisation assay of C. parvum sporozoites. The permeabilised condition used 0.1% Triton X-100 and the non-permeabilised condition used PBS. Grey (tryptophan synthase (αTrpB); green (Vicia villosa lectin); red (αCp23); blue (Hoechst). All images were taken using the same settings and exposure. Scale bar = 5μm. i. HCT8 cell monolayers were infected with either Cp23-diCRE or wildtype parasites in the presence or absence of rapamycin, and at 22 hours post infection life stages were quantified. Data shown is 2 biological replicates, n = 438 Cp23-diCRE - RAP, n = 173 Cp23-diCRE + RAP, n = 213 wildtype - RAP, n = 203 wildtype + RAP. Significance is determined using a two-tailed unpaired t-test, ns = not significant, **** = p ≤ 0.0001. j-k. Live imaging of the reinvasion event was carried out at 18 hours post infection of an HCT8 monolayer with Cp23-diCRE parasites in the presence or absence of rapamycin. Movement of merozoites was tracked using Fiji software. Representative images are shown in k. with videos in supplementary data. All live microscopy data shown is from 2 biological replicates. Significance is determined using a two-tailed unpaired t-test, **** = p ≤ 0.0001.

Journal: bioRxiv

Article Title: Targeted CRISPR Screens Reveal Genes Essential for Cryptosporidium Survival in the Host Intestine

doi: 10.1101/2024.11.22.624643

Figure Lengend Snippet: a-b. HCT8 cell monolayers infected with Cp23-diCRE in the presence or absence of rapamycin. At 6, 24 and 48 hours, gDNA was extracted and diagnostic PCRs confirmed the level of excision at the given timepoint. c. HCT8 cell monolayers infected with Cp23-diCRE in the presence or absence of rapamycin. At 6, 24 and 48 hours, the monolayer was fixed and stained and the parasite per host nuclei was quantified. Representative images are shown, with nuclei in blue (Hoechst), and parasites in green (Vicia villosa lectin). Data shown is representative of two biological replicates ± sd of the 4 technical replicates. Significance is determined using a two-tailed unpaired t-test, ns = not significant, * = p ≤ 0.05, ** = p ≤ 0.01 . d. Ifnγ -/- mice were infected with 50,000 Cp23-diCRE parasites and treated with rapamycin or DMSO control in their drinking water at 2 days post infection. Data shown is the mean of a biological replicate ± SEM of the 2 technical replicates, n = 2 mice per condition. e. Super resolution microscopy at 24 hours post infection in vitro with Cp23-diCRE parasites in the presence or absence of rapamycin; green (helix pomatia agglutinin (HPA)), parasite; magenta, (sytox), nuclei; red (Cp23 Ab), Cp23. Scale = 2μm. f. Expansion microscopy of the C. parvum asexual stages: sporozoite, merozoite, trophozoite and meront, and the sexual stages: macrogamont (female) and microgamete (male); green (helix pomatia agglutinin (HPA)); blue (N-hydroxysuccinimide (NHS ester); magenta (sytox); red (αCp23). Scale bar = 5μm. Median expansion factor of 4.5. g. Transmission electron microscopy of an excysted and unexcysted C. parvum sporozoite coupled with immunogold labelling with Cp23 antibody. Scale = 300nm . h. Permeabilisation assay of C. parvum sporozoites. The permeabilised condition used 0.1% Triton X-100 and the non-permeabilised condition used PBS. Grey (tryptophan synthase (αTrpB); green (Vicia villosa lectin); red (αCp23); blue (Hoechst). All images were taken using the same settings and exposure. Scale bar = 5μm. i. HCT8 cell monolayers were infected with either Cp23-diCRE or wildtype parasites in the presence or absence of rapamycin, and at 22 hours post infection life stages were quantified. Data shown is 2 biological replicates, n = 438 Cp23-diCRE - RAP, n = 173 Cp23-diCRE + RAP, n = 213 wildtype - RAP, n = 203 wildtype + RAP. Significance is determined using a two-tailed unpaired t-test, ns = not significant, **** = p ≤ 0.0001. j-k. Live imaging of the reinvasion event was carried out at 18 hours post infection of an HCT8 monolayer with Cp23-diCRE parasites in the presence or absence of rapamycin. Movement of merozoites was tracked using Fiji software. Representative images are shown in k. with videos in supplementary data. All live microscopy data shown is from 2 biological replicates. Significance is determined using a two-tailed unpaired t-test, **** = p ≤ 0.0001.

Techniques: Infection, Diagnostic Assay, Staining, Two Tailed Test, Control, Super-Resolution Microscopy, In Vitro, Microscopy, Transmission Assay, Electron Microscopy, Permeabilisation Assay, Imaging, Software

Comparisons of serum levels of lectin‐binding glycans between patients with HCC before operation, Day 7 after operation, and healthy individuals (means ± SD). *, p < 0.05; **, p < 0.01; ***, p < 0.001 (Mann–Whitney U test).

Journal: Cancer Medicine

Article Title: Preoperative Serum Glycan Levels Reflect Progression of Patients With Hepatocellular Carcinoma

doi: 10.1002/cam4.70285

Figure Lengend Snippet: Comparisons of serum levels of lectin‐binding glycans between patients with HCC before operation, Day 7 after operation, and healthy individuals (means ± SD). *, p < 0.05; **, p < 0.01; ***, p < 0.001 (Mann–Whitney U test).

Article Snippet: Lectins, including Aleuria aurantia lectin (AAL, L‐1390‐2), Lotus tetragonolobus lectin (LTL, L‐1320‐5), Ulex europaeus agglutinin (UEA‐I, L‐1060‐2), Lens culinaris agglutinin (LCA, L‐1040‐10), Jacalin (JAC, L‐1150‐25), Ricinus communis agglutinin I (RCA‐I, L‐1080‐10), Vicia villosa lectin (VVL, L‐1230‐5), Concanavalin A (ConA, L‐1000‐500), Narcissus pseudonarcissus lectin (NPL, L‐1370‐5), Datura stramonium agglutinin (DSA, L‐1180‐5), Wheat germ agglutinin (WGA, L‐1020‐10), PNA (L‐1070‐5), Maackia amurensis lectin I (MAL‐I, L‐1310‐5), Sambucus nigra agglutinin (SNA, L‐1300‐5), Phaseolus vulgaris Leucoagglutinin (PHA‐L, L‐1110‐5), and biotinylated NPL (B‐1375‐2) were purchased from Vector Laboratories Inc. (CA, USA).

Techniques: Binding Assay, MANN-WHITNEY

Comparison of levels of lectin‐binding glycans in preoperative serum and clinical characteristics in patients with HCC. (A) Tumor size (< 3 vs. ≥ 3 cm); (B) pTNM; (C) distant metastasis; (D) BCLC stage; (E) PLT (×10 9 /L); (F) portal hypertension (Mann–Whitney U and Kruskal–Wallis H test).

Journal: Cancer Medicine

Article Title: Preoperative Serum Glycan Levels Reflect Progression of Patients With Hepatocellular Carcinoma

doi: 10.1002/cam4.70285

Figure Lengend Snippet: Comparison of levels of lectin‐binding glycans in preoperative serum and clinical characteristics in patients with HCC. (A) Tumor size (< 3 vs. ≥ 3 cm); (B) pTNM; (C) distant metastasis; (D) BCLC stage; (E) PLT (×10 9 /L); (F) portal hypertension (Mann–Whitney U and Kruskal–Wallis H test).

Techniques: Comparison, Binding Assay, MANN-WHITNEY

Multivariate logistic analysis of lectin‐binding glycans in preoperative serum and clinical characteristics in patients with HCC.

Journal: Cancer Medicine

Article Title: Preoperative Serum Glycan Levels Reflect Progression of Patients With Hepatocellular Carcinoma

doi: 10.1002/cam4.70285

Figure Lengend Snippet: Multivariate logistic analysis of lectin‐binding glycans in preoperative serum and clinical characteristics in patients with HCC.

Techniques:

Multivariate logistic analysis of lectin‐binding glycans in postoperative serum and clinical characteristics in patients with HCC. <xref ref-type=

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Journal: Cancer Medicine

Article Title: Preoperative Serum Glycan Levels Reflect Progression of Patients With Hepatocellular Carcinoma

doi: 10.1002/cam4.70285

Figure Lengend Snippet: Multivariate logistic analysis of lectin‐binding glycans in postoperative serum and clinical characteristics in patients with HCC. ^a

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vicia villosa agglutinin vva lectinfitc (Vector Laboratories)

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